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· DNA methylation is a well-known biomarker of aging. Many previous studies have reported the change of DNA methylation patterns with age, and analyzed DNA methylation in association with aging outcomes. However, most publications were based on cross-sectional data while longitudinal evidence was largely missing an active cell death. This thesis further shows how the genome of alcoholics is deprived of DNA methylation. This is demonstrated to occur globally both in humans and in a rat model of alcohol dependence, by a technique developed during the thesis work, LUminometric Methylation Assay (LUMA), but it also occurs with gene specificity as The first three studies in this thesis focus on different mechanisms of DNA methylation related to aging, including methylation level, methylation variability and epigenetic mutation. In Study I, we investigated the longitudinal change of methylation level with age from an epigenome-wide association study (EWAS) using a mixed effect blogger.com: Yunzhang Wang
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The highly repetitive region of the BHK/PyY cell DNA is twice as methylation as the corresponding region of the BHK/C13 cell DNA. The higher level of methylation of the BHK/PyY cell DNA may therefore be due to possible reiteration of certain sequences containing 5-methyl cytosine which may simply occur less frequently in the BHK/C13 cell genome Epigenetic mechanisms, such as DNA methylation, are poorly understood in arthropods compared to mammals and plants. Arthropods have been shown to display a varietyof DNA methylation profiles across species, making them ideal for understanding the function of DNA methylation outside of a mammalian context. This thesis explores therole of DNA methylation alterations are unknown. In this thesis, I describe the results of my efforts to better understand these previously unexplored areas of biology. For the study presented in this thesis, I quantitatively profiled 95 primary prostate tumors and 86 healthy prostate tissue samples for their DNA methylation levels at 26, CpGs
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an active cell death. This thesis further shows how the genome of alcoholics is deprived of DNA methylation. This is demonstrated to occur globally both in humans and in a rat model of alcohol dependence, by a technique developed during the thesis work, LUminometric Methylation Assay (LUMA), but it also occurs with gene specificity as The aim of this thesis was to shed light on DNA methylation and its control in disease. Initially, a novel assay to estimate global DNA methylation was developed which was named LUminometric Methylation Assay (LUMA). In this assay, DNA cleavage by methylation-sensitive restriction enzymes is coupled to a polymerase Abstract. Infection of rat embryo (RE) cells with herpes simplex vims type 2 (HSV-2) inhibits the methylation of newly synthesised cellular DNA. This hypomethylation may be an imp
DNA methylation and aging : a longitudinal study of old Swedish twins
The aim of this thesis was to shed light on DNA methylation and its control in disease. Initially, a novel assay to estimate global DNA methylation was developed which was named LUminometric Methylation Assay (LUMA). In this assay, DNA cleavage by methylation-sensitive restriction enzymes is coupled to a polymerase Through six reports, the current thesis investigates DNA methylation in cancer development, by exploring this phenomenon in two cancer diseases, testicular cancer and colorectal cancer. This study shows that the two cancer types display different methylation profiles and Abstract. Infection of rat embryo (RE) cells with herpes simplex vims type 2 (HSV-2) inhibits the methylation of newly synthesised cellular DNA. This hypomethylation may be an imp
Abstract. Infection of rat embryo (RE) cells with herpes simplex vims type 2 (HSV-2) inhibits the methylation of newly synthesised cellular DNA. This hypomethylation may be an imp The aim of this thesis was to shed light on DNA methylation and its control in disease. Initially, a novel assay to estimate global DNA methylation was developed which was named LUminometric Methylation Assay (LUMA). In this assay, DNA cleavage by methylation-sensitive restriction enzymes is coupled to a polymerase The highly repetitive region of the BHK/PyY cell DNA is twice as methylation as the corresponding region of the BHK/C13 cell DNA. The higher level of methylation of the BHK/PyY cell DNA may therefore be due to possible reiteration of certain sequences containing 5-methyl cytosine which may simply occur less frequently in the BHK/C13 cell genome
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